Unknown Number 109

Pseudomonas Aeruginosa The purpose of this lab was to tell the incomprehensible bacterium agri elaboration given by our respected instructor hold various differential gear tests. The identification of this unfathomable culture was established by separating and differentiating between possible bacterium base on specific biochemical characteristics. Each test was apply in a way to help recognize those specifics and capture us one step closer to identify the unknown region cultures. The differential tests used to identify the unknown cultures were oxidase, lactose and glucose turmoil, citrate, indole, urease, motility, H2S, EMB and MAC. The tests performed on the unknown bacteria culture were wholly used to mark and identify the bacteria. Each of the tests performed provided some key information approximately the bacteria in question and how it functions. Even though I performed, all the tests listed above I will only be expand a few that I used as dete rminants concord to my dichotomous key. The oxidase test was used to test for the aim of cytochrome oxidase. The unknown bacteria was taken from the NA agar-agar monger and put on an oxidase strip in a free fall of distilled water. within seconds the bacteria will turn purple if oxidized cytochrome oxidase is position, indicating a positive test.

If no color change is notice, no cytochrome oxidase is present and the test is negative. My results for oxidase test were positive. To conduct the oxidase test I first base obtained an oxidase strip. I laid the oxidase strip on a cons titution towel. I withdrew a sample from my ! culture using and my inoculate loop and rubbed it on the strip. I waited for most 30 seconds and observed the strip turn purple. The Eosin methylene radical Blue agar-agar (EMB) is used for isolation of fecal coli form. EMB agar contains peptone, lactose, sucrose, and the dyes eosin Y and ethylene blue. The agar exhibits a potassium metallic sheen as fermentation occurs thus devising it...If you want to get a liberal essay, order it on our website: BestEssayCheap.com

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